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KMID : 0604520110370020143
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Journal of the Society of Cosmetic Scientists of Korea
2011 Volume.37 No. 2 p.143 ~ p.152
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Antioxidative Effect and Active Component Analysis of Quercus salicina Blume Extracts
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Lee Hye-Jin
Park Soo-Nam
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Abstract
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In this study, the antioxidative activity, inhibitory effects on tyrosinase and elastase, and active components of Quercus salicina Blume extracts were investigated. Q. salicina Blume was extracted using 50 % ethanol, from which ethyl acetate and aglycone fractions were prepared. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activity (FSC??) of Q. salicina Blume aglycone fraction was the highest (8.25㎍/mL). The luminol-dependent chemiluminescence assay showed that reactive oxygen species (ROS) scavenging activities (OSC??) of Q. salicina Blume aglycone fraction on ROS generated in Fe³＋－EDTA/H₂O₂ system was the most prominent at 0.039㎍/mL. The protective effects of extract/fractions of Q. salicina Blume against the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1~25㎍/mL). Especially, τ?? of aglycone fraction in 10㎍/mL concentration showed the most protective effect at 259.9 min. The inhibitory effects (IC??) on tyrosinase and elastase of Q. salicina Blume extracts were higher at aglycone fraction (respectively, 21.82㎍/mL, 41.18㎍/mL). Active component analysis by TLC and HPLC showed quercetin, keampferol, catechin, quercitrin, isoquercitrin, hyperoside, and etc. These results indicate that Q. salicina Blume extract has strong antioxidative activity and can be used as antioxidant. Particularly, aglycone fraction of Q. salicina Blume showed superior antioxdative activity and high inhibitory effect on tyrosinase and elastase. Therefore, aglycone fraction of Q. salicina Blume could be applicable to new functional cosmetics.
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KEYWORD
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Quercus salicina Blume, antioxidative activity, flavonoid, cosmetics
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